Research

We have a very broad interest in infectious disease. Currently the Lab has multiple active projects, mostly in collaboration with different research groups across US. One project is to identify immunity related long non-coding RNAs and epigenetic changes, using integrative approaches including custom RNA-seq analysis and CRISPR/Cas9 based library screen. Another project is to study the impact of microbiome on host immunity like SIV vaccine efficacy in macaques. We are interested in novel approaches that can go deeper than standard 16S based sequencing and examine microbiome functional activities. The other active project is the genetics and transcriptional regulation of complex immune genes such as macaque MHC and KIR genes and Fc gamma receptors in human and macaque. Our group is tackling this complexity with the development of new computational methods combined with different sequencing technologies like PacBio’s long reads. Lastly, we are actively building collaborative researches in other infectious disease areas like influenza infection and vaccine development and antimicrobial resistance. Our research is supported by NIH and the College’s startup fund.

Immunofluorescence Assay (NL4-3)


SUPT1 cells are infected with HIV strain NL4-3 and stained for viral capsid (core) protein using anti P24-GFP monoclonal antibody. Note cytopathological giant cell formation.

Immunofluorescence Assay (LAI)


SUPT1 cells are infected with HIV strain LAI and stained for viral capsid (core) protein using anti P24-GFP monoclonal antibody. We use this assay to verify infection prior to downstream applications.